Use of 59Fe Isotope in Iron Chlorosis for Fodder Sorghum Bicolor

The effect of foliar spray of different chemicals on the sorghum cultivar KH-333 in four type nutrient medium viz; normal pH, high pH, high bicarbonate (10 mM) and, high pH (8.0) &high bicarbonate (10 mM) on biochemical parameters and the recovery of iron chlorosis in fodder sorghum was examined. The effect of iron chlorosis and sulphydryl compounds on the antioxidant enzymes and active iron content to see whether sorghum plants are able to cope up with iron stress by foliar treatment with iron compounds and thio biomolecules. The activity of some enzymes involved in antioxidant defense and thiols associated mechanism has been studied. The activity of antioxidant defense system related enzymes (APOX, GPOX, CAT and SOD) were found higher by foliar spray of 0.2% FeSO4 and chelating agents. Activity of active iron content was recorded higher with treatment of iron and thiol compounds. In another experiment, Fe absorption and transport was measured over 72 hour, using 59Fe labelled 0.01mM FeSO4 , 0.05 mM FeSO4 , 0.01 mM Fe-EDDHA and 0.05 mM Fe-EDDHA(pH 6.3). The plant parts (Root and shoot) were radio assayed in a liquid scintillation counter. The absorption and transport of Fe from FeSO4 are found to be higher than from FeEDDHA in plant (root and shoot). It is also shown that very less quantity is transported to the shoot.